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Gel of RT PCR with SPH-1 primers

TOP GEL Lanes 1 and 8: 5 uL Low Mass ladder (https://www.lifetechnologies.com/order/catalog/product/10068013) Lanes 2, 3, 4, and 5: 3 uL Negative Controls + 1 uL...

A primer specific for SPH-1

I designed a primer for the gene with this accession number: CP000884.1, annotated as a hypothetical protein–http://www.ncbi.nlm.nih.gov/nuccore/160361034?from=1899744&to=1900880&sat=4&sat_key=103985021 Here is the primer designed by Primer-BLAST:   All...