Gel of RT PCR with Cs1-4 gDNA and Primer Set D. Cs1-4 #1

RTPCR CS14 2 2014-07-25 13hr 32min

 

Predicted product size for reaction: 400 bp

Top gel:

Lane 1– Low mass ladder. First three bands from bottom are 100 bp, 200 bp, 400 bp

Lanes 2-5– 2 µL reaction negative control + 1 µL loading dye + 7 µL water

Lanes 6-7– 2 µL reaction with 0.01 ng + 1 µL loading dye + 7 µL water

 

Bottom gel:

Lane 1– Low mass ladder. First three bands from bottom are 100 bp, 200 bp, 400 bp

Lanes 2-3– 2 µL reaction with 0.1 ng + 1 µL loading dye + 7 µL water

Lanes 4-5– 2 µL reaction with 1 ng + 1 µL loading dye + 7 µL water

Lanes 6-7– 2 µL reaction with 10 ng + 1 µL loading dye + 7 µL water

 

All reactions are predicted size.

 

 

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