A primer specific for SPH-1

I designed a primer for the gene with this accession number: CP000884.1, annotated as a hypothetical protein–http://www.ncbi.nlm.nih.gov/nuccore/160361034?from=1899744&to=1900880&sat=4&sat_key=103985021

Here is the primer designed by Primer-BLAST:



All the primers on the genome:

Primer on genome


Blasting the amplicon:


Blasting the amplicon


The amplicon:


Blast results of the forward primer and the first few hits:

Blast result of forward primer

Blast of Forward Primer

Blast results of the reverse primer and first few hits:

Blast result of reverse primer

Blast of reverse primer


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