I designed a primer for the gene with this accession number: CP000884.1, annotated as a hypothetical protein–http://www.ncbi.nlm.nih.gov/nuccore/160361034?from=1899744&to=1900880&sat=4&sat_key=103985021
Here is the primer designed by Primer-BLAST:
All the primers on the genome:
Blasting the amplicon:
The amplicon:
Blast results of the forward primer and the first few hits:
Blast results of the reverse primer and first few hits:
