Gel of RT PCR with SPH-1 primers

TOP GEL

Lanes 1 and 8: 5 uL Low Mass ladder (https://www.lifetechnologies.com/order/catalog/product/10068013)

Lanes 2, 3, 4, and 5: 3 uL Negative Controls + 1 uL loading dye + 6 uL water

Lanes 6 and 7: 3 uL RT PCR reaction with 0.01 ng/uL D. acidovorans SPH-1 DNA + 1 uL loading dye + 6 uL water

BOTTOM GEL

Lanes 1 and 8: 5 uL Low Mass ladder (https://www.lifetechnologies.com/order/catalog/product/10068013)

Lanes 2 and 3: 3 uL RT PCR reaction with 0.1 ng/uL SPH-1 DNA + 1 uL loading dye + 6 uL water

Lanes 4 and 5: 3 uL RT PCR reaction with 1 ng/uL SPH-1 DNA + 1 uL loading dye + 6 uL water

Lanes 6 and 7: 3 uL RT PCR reaction with 10 ng/uL SPH-1 DNA + 1 uL loading dye + 6 uL water

RT PCR 2014-07-16

 

All DNA was amplified at the correct size (~100 bp), but DNA was also amplified in negative controls.

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