Predicted product size for reaction: 400 bp
Top gel:
Lane 1– Low mass ladder. First three bands from bottom are 100 bp, 200 bp, 400 bp
Lanes 2-5– 2 µL reaction negative control + 1 µL loading dye + 7 µL water
Lanes 6-7– 2 µL reaction with 0.01 ng + 1 µL loading dye + 7 µL water
Bottom gel:
Lane 1– Low mass ladder. First three bands from bottom are 100 bp, 200 bp, 400 bp
Lanes 2-3– 2 µL reaction with 0.1 ng + 1 µL loading dye + 7 µL water
Lanes 4-5– 2 µL reaction with 1 ng + 1 µL loading dye + 7 µL water
Lanes 6-7– 2 µL reaction with 10 ng + 1 µL loading dye + 7 µL water
All reactions are predicted size.