Jul 17, 2014
Gel of RT PCR with SPH-1 primers
TOP GEL Lanes 1 and 8: 5 uL Low Mass ladder (https://www.lifetechnologies.com/order/catalog/product/10068013) Lanes 2, 3, 4, and 5: 3 uL Negative Controls + 1 uL loading dye + 6 uL water Lanes 6 and 7: 3 uL RT PCR reaction with 0.01 ng/uL D. acidovorans SPH-1 DNA + 1 uL loading dye + 6 uL water BOTTOM…