Aug 8, 2014

Protocols for 16S, Biofilm gDNA Extraction from Swab, and RT PCR Limit Detection Assay

Jul 30, 2014

Gel of RT PCR with Cs1-4 gDNA and Primer Set D. Cs1-4 #1

  Predicted product size for reaction: 400 bp Top gel: Lane 1– Low mass ladder. First three bands from bottom are 100 bp, 200 bp, 400 bp Lanes 2-5– 2 µL reaction negative control + 1 µL loading dye + 7 µL water Lanes 6-7– 2 µL reaction with 0.01 ng + 1 µL loading…

Jul 17, 2014

Gel of RT PCR with SPH-1 primers

TOP GEL Lanes 1 and 8: 5 uL Low Mass ladder (https://www.lifetechnologies.com/order/catalog/product/10068013) Lanes 2, 3, 4, and 5: 3 uL Negative Controls + 1 uL loading dye + 6 uL water Lanes 6 and 7: 3 uL RT PCR reaction with 0.01 ng/uL D. acidovorans SPH-1 DNA + 1 uL loading dye + 6 uL water BOTTOM…

Jul 14, 2014

PCR with Genus and Strain Specific Primers on SPH-1 DNA Template

  Lane 1: 10 ul 1 kb Hyperladder Lane 2: PCR reaction with D.G #1 Lane 3:  PCR reaction with D. a. SPH-1 #2 Lane 4: D. Cs1-4 #1 Lane 5: D. a. SPH-1 #2 Lane 6: negative control Lane 7: Empty Lane 8: 5 uL 1 kb Hyperladder  

Jul 10, 2014

Image of 16S PCR with ATCC 15668 and SPH-1 and Negative Contr

For cross reference, since the image on page 17 of Aruna’s lab notebook does not show the negative control band clearly.  

Jun 30, 2014

A primer specific for SPH-1

I designed a primer for the gene with this accession number: CP000884.1, annotated as a hypothetical protein–http://www.ncbi.nlm.nih.gov/nuccore/160361034?from=1899744&to=1900880&sat=4&sat_key=103985021 Here is the primer designed by Primer-BLAST:   All the primers on the genome:   Blasting the amplicon:     The amplicon: Blast results of the forward primer and the first few hits: Blast results of the reverse primer…